Flexible implementation of modulated localization microscopy based on DMD

Abigail Illand (1) , Pierre Jouchet (1) , Emmanuel Fort (2) , Sandrine Lévêque-Fort (1)

1 ISMO - Institut des Sciences Moléculaires d'Orsay
2 IL - Institut Langevin - Ondes et Images (UMR7587)

Localization microscopy of individual molecules allows one to bypass the diffraction limit, revealing cellular organization on a nanometric scale. This method, which relies on spatial analysis of the signal emitted by molecules, is often limited to the observation of biological objects at shallow depths, or with very few aberrations. The introduction of a temporal parameter into the localization process through a time modulated excitation was recently proposed to address these limitations. This method called ModLoc, is demonstrated here with an alternative flexible strategy. In this implementation, to encode the time modulated excitation a digital micromirror device is used in combination with a fast demodulation approach, and provides a twofold enhancement in localization precision.

Keywords: single molecule localization structured excitation fluorescence microscopy DMD; single molecule localization; structured excitation; fluorescence; microscopy; DMD

Domains: Physics [physics]; Optics / Photonic

Abigail Illand, Pierre Jouchet, Emmanuel Fort, Sandrine Lévêque-Fort. Flexible implementation of modulated localization microscopy based on DMD. 2023. ⟨hal-04419833⟩